Table 3.
Deracemization of 2-hydroxy acids with the mixtures of E. coli BL21(DE3)/pET28b-HADH and E. coli BL21(DE3)/pCDFDuet-KAR-GDH
| Entry | Substrate | Reaction time (h) | Conversion (%) | ee of (R)-1 (%) |
|---|---|---|---|---|
| 1 | rac-1a | 4 | 94.9 | >99 |
| 2 | rac-1b | 6 | 94.5 | >99 |
| 3 | rac-1c | 6 | 93.2 | >99 |
| 4 | rac-1d | 4 | 95.1 | >99 |
| 5 | rac-1e | 4 | 96.4 | >99 |
| 6 | rac-1f | 4 | 97.3 | >99 |
| 7 | rac-1g | 4 | 97.6 | >99 |
| 8 | rac-1h | 2 | 95.5 | >99 |
| 9 | rac-1i | 4 | 96.8 | >99 |
| 10 | rac-1j | 2 | 94.5 | >99 |
| 11 | rac-1k | 4 | 96.3 | >99 |
| 12 | rac-1l | 2 | 96.4 | >99 |
| 13 | rac-1m | 4 | 94.8 | >99 |
| 14 | rac-1n | 6 | 35.5 | 81.9 |
| 15 | rac-1o | 6 | 34.6 | 71.8 |
| 16 | rac-1p | 6 | 59.5 | >99 |
| 17 | rac-1q | 6 | 42.7 | >99 |
| 18 | rac-1r | 6 | 76.4 | 63.3 |
| 19 | rac-1s | 6 | 58.3 | 60.1 |
Reactions were performed in one pot at 35 °C in phosphate buffer (100 mM, pH 7.5) with 4 g DCW/L resting cells of E. coli BL21 (DE3)/pET28b-HADH, 8 g DCW/L resting cells of E. coli BL21 (DE3)/pCDFDuet-KAR-GDH and 20 mM substrate