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. 2016 Sep 22;16:746. doi: 10.1186/s12885-016-2776-1

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Hypoxia dampens MTX and PRD effect on leukemic cells viability without affecting viable cell density. a Effect of hypoxia (5 % O₂) on viable cell density of leukemic cells treated with several concentrations of MTX or PRD at 72 h. b Effect of hypoxia (5 % O₂) on viability of leukemic cells treated with several concentrations of MTX or PRD at 72 h. Viable cell density and percentage of cell viability measurement were performed by an automatic cell counter. All results are representatives with mean ± S.E.M. of 4 independent experiments. *indicates p < 0.05; **p < 0.01; ***p < 0.001 vs normoxia (21 % O₂)