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. 2016 Sep 22;16:746. doi: 10.1186/s12885-016-2776-1

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Hypoxia inhibits MTX-induced cell death pathways in Nalm-6 cells. a Effect of several concentrations of MTX over 24 h on Nalm-6 cells maintained in normoxia (21 % O₂) or in hypoxia (5 % O₂) on 10 pro-apoptotic proteins. b Effect of several concentrations of MTX over 24 h on Nalm-6 cells maintained in normoxia (21 % O₂) or in hypoxia (5 % O₂) on 3 anti-apoptotic proteins. Proteomic analysis was performed by RPPA. Data are represented by experimental conditions and by protein expressions after Z-score normalization. Protein expressions with two standard deviations away from the mean were analyzed