Fig. 1.

FACS analysis of neurotrophic tyrosine kinase receptor type 2 (TRKB) expression in cultured human exocrine tissue. Single cells from a primary suspension culture of adult human exocrine tissue were stained with anti-TRKB and anti-mouse Alexa Fluor-647 then fixed, permeabilized and stained with anti-NGN3 (a-d). a NGN3+ population gated based on isotype negative control. b TRKB/NGN3 coexpression of population gated in a. c TRKB+ population gated based on isotype negative control. d TRKB/NGN3 coexpression of population gated in c. Single cells from a biological replicate exocrine tissue culture were stained with anti-TRKB and anti-mouse Alexa Fluor-647 then stained with anti-CD133-PE (phycoerythrin) (e-h). e CD133+ population gated based on isotype negative control. f TRKB/CD133 coexpression of population gated in e. g TRKB+ population gated based on isotype negative control. h TRKB/CD133 coexpression of population gated in g. Antibody combination shown above plots. Gates shown with dashed and solid red lines. Percentage of cells in each gate are shown in upper corner