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. 2016 Aug 11;5(10):844–857. doi: 10.1016/j.molmet.2016.08.003

Figure 3.

Figure 3

α-MSH signaling does not mediate the stimulatory effects of kisspeptin on puberty/gonadotropic axis. (A) The effects of intracerebroventricular (icv) injection of an effective dose (1 nmol) of the MC3/4R antagonist SHU9119 on basal LH secretion and stimulated responses to MT-II and Kp-10 are presented (n = 9–10/group). While SHU9119 decreased basal LH levels and fully prevented LH responses to the MC3/4R agonist MT-II in pubertal male rats, it did not affect LH responses to a submaximal (50 pmol) dose of Kp-10. (B) A summary of phenotypic and hormonal indices from a mouse model of congenital elimination of kisspeptin receptor Gpr54 from POMC neurons is shown. Neither VO (as external sign of puberty) nor the age of first estrus or the magnitude of LH responses to Kp-10 were altered in mice with congenital ablation of Gpr54 in POMC neurons. Note that basal LH levels were not altered and are denoted as dotted line (right panel). *** P < 0.001 vs. corresponding time-points in vehicle-injected animals (Student t-test or ANOVA followed by Student–Newman–Keuls multiple range test).