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. 2016 Aug 5;5(10):869–881. doi: 10.1016/j.molmet.2016.07.012

Figure 5.

Figure 5

Serial plasma leptin levels and functional measurements of leptin sensitivitity in ArcPomc−/−;CreERT mice. A) Serial plasma leptin levels at the indicated experimental stages; ****P < 0.0001, ArcPomc+/+;Cre-ERT vs. ArcPomc−/− and ArcPomc−/−;Cre-ERT and ####P < 0.0001 for ArcPomc−/− vs. ArcPomc+/+;Cre-ERT and ArcPomc−/−;Cre-ERT for males (n = 6–10) and females (n = 8–10), Tukey's multiple comparisons tests following separate 1-way ANOVAs for each combination of sex and experimental stage. B) 24-h changes in body weight and C) Absolute 24-h food intake after i.p. leptin treatment at the indicated experimental stages; ****P < 0.0001, ∗∗∗P < 0.005 for leptin vs. saline treated ArcPomc+/+;Cre-ERT for both sexes; ####P < 0.0001, ###P < 0.005 for leptin vs. saline treated ArcPomc−/−;Cre-ERT for both sexes, paired 2-tailed Student's t-tests, n = 5–8. Data shown are the mean ± SEM. WM, weight matched; Post-TAM, post-tamoxifen. For simplicity, ArcPomc−/−;Cre-ERT is used as a common annotation for either ArcPomc−/−;CAG-Cre-ERT or ArcPomc−/−;Pomc-Cre-ERT2 compound mice.