Supplemental Figure 1.

Solution change, vehicle-only, and pharmacological drug-only controls. (A) Pooled data scatter plot of %ΔIR of VL-VMN neurons during control solution change from both females (n = 13) and males (n = 6). (B) Quantification of %ΔIR of VL-VMN neurons in response to vehicle controls pooled from both sexes. Numbers above columns indicate the n for each group. (C, D) Quantification of %ΔIR in response to MPP, PHTPP, AICAR and CC in 2.5 mM glucose for nonadapting GI (C) and AdGI neurons (D) from females only. Numbers above columns indicate the n number for each group. Shaded boxes represent 2xSTD of control solution change experiments (see Supplemental Figure 1A). Treatments exhibiting %ΔIR responses within this shaded area were deemed as “no effect.” *p < 0.05 via one-sample student t-test with a theoretical mean = 0. AICAR: AMPK agonist (0.5 mM), CC: AMPK antagonist (10 μM), G: mM glucose, ER: estrogen receptor, IR: input resistance, MPP: ERα antagonist (10 μM), PHTPP: ERβ antagonist (1 μM), Res: 2.5G from general reservoir, Syr: 2.5G from treatment syringe, Vm: membrane potential.