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. 2016 Sep 22;11:126. doi: 10.1186/s13014-016-0703-x

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© The Author(s). 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Checking of PARP-1 expression by IF study. a Typical photographs of immunostained H-vector & HsiI cells are given. Anti-PARP-1 antibody was detected by the secondary antibodies of FITC tagged goat anti-mouse IgG₁. DAPI was used to counterstain the nucleus. Scale bar represents 20 μm. b Relative expression of PARP-1 was calculated with respect to beta actin by measuring fluorescence intensities by ImageJ software. PARP-1 expression in H-vector was plotted as 100 % protein expression and accordingly PARP-1 level in HsiI was calculated and plotted. This experiment was repeated four times. ‘###’ represents p ≤ 0.001