Fig. 7.
WNT-induced dynamic mass redistribution depends on Gα12/13 and FZD4-GFP. HEK293 wild-type (wt) cells and HEK293 cells lacking Gα12/13 were stimulated with increasing amounts of WNT-5A (100, 300, and 1000 ng/ml). Changes in DMR were recorded over time. The apparent negative, WNT-5A–induced DMR responses in empty vector (A) or FZD4-GFP–transfected (B) HEK293 cells were not observed in the absence of Gα12/13 proteins (C and D). Experiments were done after overnight treatment with the porcupine inhibitor C59 (5 µM) at 37°C. Shown are representative traces (N = 3), buffer-corrected and measured in triplicates + S.E.M. See Supplemental Fig. 5 for expression levels of FZD4-GFP and forskolin-induced DMR responses of wt and Gα12/13-knockout (KO) HEK293 cells.