Supplemental Figure 2.

Over-expressing dominant negative variants of MRTFA or SRF in C3H10T1/2 cells inhibited osteogenesis but enhanced adipogenesis. Post-confluent C3H10T1/2 cell lines over-expressing DN-MRTFA, DN-SRF, and controls were treated with osteogenic inducing media for 21 days. The RNA was then extracted from these cells for RT-PCR experiments to measure the fold change of osteoblastic genes in A and B. Data are expressed as mean ± SEM (n = 3). *p < 0.05.