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. 2016 Aug 26;5(10):970–979. doi: 10.1016/j.molmet.2016.08.012

Figure 4.

Figure 4

Bone marrow stromal cells from MRTFA KO mice showed enhanced adipogenic but reduced osteoblastic differentiation. 12 weeks old WT and MRTFA KO mice were euthanized, and their femur and tibia bone marrow cavity contents were flushed out with αMEM growth media containing 10% fetal bovine serum (FBS). Cells were cultured with osteogenic inducers for 21 days until fully differentiated (A–C). Light microscopy images of osteogeneic cultures at day 21 (scale bar = 0.3 mm) (A). Osteogenic gene mRNA expression of WT and KO osteogeneic cultures (B). Alizarin Red S staining of WT and KO cells visualizing mineral deposition during osteogenesis. Bone marrow derived cells were cultured with adipogenic inducers for 10 days (D–F). Light microscopy images of adipogenic cultures at day 10 (scale bar = 0.3 mm) (E). Cells were then harvested for western blot and RT-PCR analysis. Adiponectin protein expression and mRNA expression of adipogenic makers (E–F) of WT and KO cells. Data are expressed as mean ± SEM (n = 3). *p < 0.05; **p < 0.01.