Table 1.
MRTFA KO female mice showed an osteopenic phenotype as compared to the WT mice. Micro-CT analysis of bone parameters in cortical diaphysis and trabecular region of WT and MRTFA KO mice femurs are shown. 14 female mice (WT n = 7, KO n = 7) at the age of 24 weeks were analyzed for this study. Whole body weight, length of femurs and tibiae of WT and MRTFA KO mice are also shown (*p < 0.05, **p < 0.01).
| WT (n = 7) | KO (n = 7) | p-Value | |
|---|---|---|---|
| Body weight (g)* | 28.2 ± 3.2 | 24.7 ± 3.4 | 0.04 |
| Femur length (mm)** | 15.6 ± 0.5 | 15.0 ± 0.5 | 0.006 |
| Tibia length (mm)** | 18.2 ± 0.4 | 17.5 ± 0.4 | 0.0003 |
| Cortical total volume (TV) (mm³)** | 0.50 ± 0.04 | 0.44 ± 0.05 | 0.01 |
| Cortical bone volume (BV) (mm³)** | 0.48 ± 0.03 | 0.43 ± 0.05 | 0.01 |
| Cortical BV/TV (1) | 0.98 ± 0.009 | 0.98 ± 0.001 | 0.41 |
| Cortical structure model index (1)** | 0.70 ± 0.03 | 0.61 ± 0.05 | 0.0002 |
| Cortical thickness (mm)** | 0.22 ± 0.009 | 0.19 ± 0.013 | 0.00001 |
| Cortical mean/density of TV (apparent) (mgHA/ccm)** | 1412 ± 9 | 1384 ± 20 | 0.0003 |
| Cortical mean/density of BV (material) (mg HA/ccm)** | 1531 ± 9 | 1513 ± 17 | 0.004 |
| Trabecular total volume (TV) (mm³) | 1.42 ± 0.1 | 1.37 ± 0.1 | 0.18 |
| Trabecular bone volume (BV) (mm³)** | 0.05 ± 0.1 | 0.02 ± 0.01 | 0.004 |
| Trabecular BV/TV (1)** | 0.04 ± 0.01 | 0.02 ± 0.007 | 0.004 |
| Connectivity density (1/mm³)* | 8.2 ± 6.3 | 2.1 ± 1.9 | 0.01 |
| Structure model index (1) | 3.72 ± 0.2 | 3.84 ± 0.2 | 0.14 |
| Trabecular number (1/mm)* | 2.80 ± 0.4 | 2.23 ± 0.6 | 0.02 |
| Trabecular thickness (mm) | 0.052 ± 0.01 | 0.048 ± 0.01 | 0.25 |
| Trabecular separation (mm)** | 0.36 ± 0.05 | 0.43 ± 0.04 | 0.006 |
| Trabecular mean/density of TV (apparent) (mg HA/ccm)** | 123 ± 16 | 98 ± 18 | 0.009 |
| Trabecular mean/density of BV (material) (mg HA/ccm) | 1032 ± 31 | 1017 ± 76 | 0.32 |