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ROS ratiometric probe design and characterization. (A) Upon exposure to ROS, L-012-and-NIR NPs display chemiluminescence. (B) Nanoparticles are uniformly spherical with an average diameter of 25 nm. (C) FTIR analysis displays bare nanoparticle makeup as a combination of hydrophilic PEG domains and hydrophobic polystyrene ones. (D) An MTT assay showed no significant cytotoxicity of dye-loaded NPs to 3T3 cells.
