Table 2. Loss of function of specific genes was predicted to arrest the cell cycle at different phases.

Mutant	Prediction	Experimental Support	Reference (SGD ID)
Cln3	Prevents transition from Start to G1	Incorrect prediction: Cln3 knockdown is found to lead to increased G1 duration	S000000038
MBF or SBF	Prevents transition from G1 to S	Either MBF or SBF knockdown leads to increased g1 duration	S000004172
Clb56 or SBF	Prevents transition from S to G2	Clb56 knockdown causes a delay in the progression through S phase	S000006324
Clb12 or SBF	Prevents transition from G2 to M	(1) SBF knockdown leads to delayed G2/M transition	(1) S000000913
		(2) Clb12 knockdown delays progression through M phase	(2) S000006323
Cdc20	Prevents transition from M to G0	Cdc20 knockdown delays progression through M phase	S000003084

Experimental support for these predictions has been found through the Saccharomyces Genome Database (www.yeastgenome.org). Only one prediction was found to be incorrect (Cln3 mutant).