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. Author manuscript; available in PMC: 2016 Sep 23.
Published in final edited form as: J Gen Virol. 2011 Jun 29;92(Pt 10):2437–2445. doi: 10.1099/vir.0.033670-0

Fig. 6.

Fig. 6

Detection of E5 mRNA in PBMCs by RT-PCR. A 499 bp fragment of the E5 gene was detected by RT-PCR, as described in Methods. +RT, Reverse transcription of mRNA with enzyme; –RT, no-enzyme control; L, 100 bp DNA ladder (Fermentas); +C, sarcoid DNA (positive control); –C, virus-free equine skin DNA (negative control).