Table 1.
miRNAs differentially regulated by DHA, fiber, or curcumin in the colon
| Bioactive component | Differentially regulated genes | Validated mRNA targets | Cell line/organism | Target mRNA-involved pathway | Reference |
|---|---|---|---|---|---|
| n-3 PUFA | miR-18a | Runx1 | Rat colon | ERK-MAPK, Wnt/β-catenin, PTEN, apoptosis, EMT | 190 |
| miR-19b | Arid4b, Arpc3, Hipk3 | ||||
| miR-27b | Cxcl12, Runx1 | ||||
| miR-93 | Sqstm1, Stat3, Vegfa | ||||
| miR-497 | Bcl2, Bcl2ls, Eln | ||||
| miR-132 | Arhgap32, Btg2, Ep300, Foxo3, Kdm5a, Mecp2, Mmp9, Nr4a2, Paip2, Ptbp2 | ||||
| miR-146b | Nfkb1, Sirt1 | ||||
| miR-192 | Zeb2 | ||||
| miR-206 | Bdnf, Fstl1, Gja1, Hdac4, Hhip, Id1, Id2, Id3, Igsf5, Mmd, Notch3, Pax7, Pola1, Ptplad1, Spry1, Timp3, Utrn | ||||
| miR-218 | Onecut2 | ||||
| let-7d | Sept3 | 46 | |||
| miR-15b | Bcl2, Ccne1 | ||||
| miR-107 | Bace1, Serbp1 | ||||
| miR-191 | Mxi1, Riok3 | ||||
| miR-324 | Smo | ||||
| miR-1 | Calm1, Calm2, Gata4, Hdac4, Igf1, Mef2a | Human colorectal adenocarcinoma cells | EMT, cancer cell migration, invasion, tumor suppressor | 72 | |
| miR-30c | Ccne2, Celsr3, Egfr, HSPA4, Mdm2, Mtdh, Runx1, Smad1, Snai1, Timp3, Twf1, Vimentine | ||||
| miR-141 | Cdh11, Dlx5, Elavl4, Klf5, Mapk14, PTEN, Slc25a3, Stk3, Zeb1, Zeb2 | ||||
| miR-181a | Acvr2a, Bcl2, Cbx7, Cd69, Egr1, Gata6, Hoxa, Msx2, Rgs4, Runx1, Tbr1, Tcra, Tgfbi, Tox | ||||
| miR-192 | Zeb2 | ||||
| miR-221 | Arnt, Bnip3, Cdkn1b, Ddit4, Kit, Psmd9, Timp3 | ||||
| miR-1283 | |||||
| let-7f | |||||
| miR-15b | Arl2, Bcl2l2, Dedd | Human gastric cancer cells | 202 | ||
| miR-16 | App, Arl2, Bcl2, Bcl2l2, Cadm1, Ccnd1, Ccne1, Ccnt2, Cd40, Fgf2, Jag1, Jun, Mdm4, Slc6a4, Vegfa,Wnt3a | ||||
| n-3 PUFAs plus pectin | miR-16 | Arl2, App, Bcl2, Ccnd1, Jag1, Jun, Mdm4, Vegfa, Wnt3a | Rat colon | Adenocarcinoma, mTOR, PI3K/AKT, apoptosis, EMT | 190 |
| miR-21 | Eif4e3, Fasl, Peli1, Pdcd4, Pten, Reck, Smad7, Spry1, Spry2, Tgfbr3, Tnfaip8l2, YOD1, Yy1 | ||||
| miR-26b | Lef1, PDE4B | ||||
| miR-27b | Cxcl12, Mef2c, Pparg, Runx1, Smad3, Smad4, Smad5 | ||||
| miR-30b | Aicda, Snai1 | ||||
| miR-93 | Sqstm1, Stat3, Vegfa, VSIVgp2 | ||||
| miR-98 | Acvr1b, Il6, Mmp11 | ||||
| miR-130b | Meox2, Tgfb1 | ||||
| miR-182 | Adcy6, Clic5, Fbxw7, Foxo3, Tbx1 | ||||
| miR-200c | Bmi1, Flt1, Mapk14, Nog, Reln, Sox2, Vldlr, Zeb1, Zeb2, Zfpm2 | ||||
| miR-203 | Cav1, Ctnnb1, Rbm44, Snora62, Trp63, TCF4, Vcan, ZNF148 | ||||
| miR-206 | Adar, Bdnf, Clcn3, Eif4e3, Fn1, Fstl1, Fzd7, Gja1, Hdac4, Hhip, Hmgb3, Id1, Id2, Id3, Igsf5, Meox2, Mmd, Nfat5, Notch3, Pax7, Pdcd10, Pola1, Ptplad1, Rarb, Sh3bgrl, Spry1, Smarcb1, Smarcd2, Timp3, Utrn | ||||
| Curcumin | mir-21 | AP-1, Eif4e3, Fasl, Pdcd4, Peli1, Pten, Reck, Smad7, Spry1, Spry2, Tgfbr3, Tnfaip8l2, YOD1, Yy1 | Human colorectal adenocarcinoma cells | Apoptosis, proliferation, inflammation, Wnt, EGFR | 141 |
| mir-34a | Actb, Bcl2, Bcl6, Ccnd1, Dll1, Foxp1, Gas1, Notch-1, Pofut1, Ppp1r10, Sema4b, Sirt1, TGIF2, Trem2, Vcl, Vegfb | Human esophageal cancer cells | 200 | ||
| mir-17 | Specificity protein transcription factors, Bcl2l11, Mapk14, Rbl2, Sqstm1, Stat3, Tgfbr2 | Human colon carcinoma cell | 65 | ||
| mir-20a | App, Bmp4, Ep300, Fgf10, Hbp1, Mapk14, Osr1, Pten, Shox2, Sqstm1, Stat3, Tbx3, Tgfbr2, Trp53inp1, ULK1, Vegfa, Zbtb7a, Zfpm2 | ||||
| mir-27a | Alp, Bmp2, Bmpr1a, Creb1, Fbxw7, Il10, Odc1, Ppara, Pparg, Prdm16, Runx1, Runx2, Smad9, Spp1, Srm, Tcirg1 |
Abbreviations: EGFR, epidermal growth factor receptor; EMT, epithelial-mesenchymal transition; ERK, extracellular-signal-regulated kinase; MAPK, mitogen-activated protein kinase; PTEN, phosphatase and tensin homolog deleted on chromosome 10; PUFAs, polyunsaturated fatty acids.