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. 2016 Sep 23;11(9):e0163633. doi: 10.1371/journal.pone.0163633

Fig 4. 19(S)-HETE is a selective IP receptor agonist.

Fig 4

(A) COS-1 cells expressing Gq/G11-coupled prostanoid receptors together with a Ca2+-sensitive bioluminescent fusion protein were exposed to 19(S)-HETE (3 μM) and their cognate prostanoid receptors ligands at 3 μM. EP1, PGE2 receptor subtype 1; FP, prostaglandin F2α receptor; TP, thromboxane A2 receptor. AUC, area under the curve of agonist-induced calcium transients recorded for 100 seconds. (B) Effect of 19(S)-HETE (3 μM) on various Gi-coupled prostanoid receptors expressed together with the promiscuous G-protein α-subunit Gα15 in COS-1 cells using a Ca2+-sensitive bioluminescent probe. Functionality of receptors was verified by recording responses after stimulation with the specific prostanoid receptors at 3 μM. EP3, PGE2 receptor, subtype 3; DP2, prostaglandin D2 receptor. (C) Gs-coupled prostanoid receptors were heterologously expressed together with a cAMP-sensitive bioluminescence probe and stimulated with 3 μM of 19(S)-HETE or their endogenous ligands. EP2 and EP4, prostaglandin E2 receptors, subtypes 2 and 4; DP1, prostaglandin D1 receptor; IP, prostacyclin receptor. Luminescence refers to the light generated 15 minutes after ligand stimulation and recorded with an integration time of 1250 ms.