Fig 4. Neocentromere chromosome loss rate correlates with transcriptional activity, but not chromosomal position.

A. The fold-difference in URA3 loss rate between the mean rate for the native centromere strain and the mean rate of each neocentromere strain was plotted as a function of the fraction of the neocentromere CENP-A bound region that includes ORFs multiplied by the RNA-seq transcriptional measurement on a log2 scale of RPKM. Correlation between these two variables was high (r² = 0.71). B. The fold-difference in URA3 loss rate between the mean rate for the native centromere strain and the mean rate of each neocentromere strain was plotted as a function of the distance between the neocentromere position and the native centromere. Correlation between these two variables was very low (r² = 0.06).