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. 2016 Sep 23;11(9):e0163580. doi: 10.1371/journal.pone.0163580

Fig 4. Differentiation of iTS-P into insulin-producing cells.

Fig 4

(A) RT-PCR analysis for mRNA expression of pancreatic β-cell markers in differentiated iTS-P cells (iTS-P 4–2 Stages 1–5 and iTS-P 4–2 Stages 4–5) and ES cells (Stages 1–5 ES and Stages 4–5 ES). Isolated islets were used as the positive control. Abbreviations: Nkx2.2, NK2 homeobox 2; Nkx6.1, NK6 homeobox 1; Pax4, paired box protein 4; Pax6, paired box protein 6; Ins1, insulin 1; Ngn3, neurogenin 3; NeuroD, neurogenic differentiation. (B) Quantitative RT-PCR analysis of insulin-1 (upper panel) and -2 (lower panel) mRNA in differentiated iTS-P and ES cells. Isolated islets were used as the positive control. *P<0.05 between two groups.