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. 2016 Sep 23;11(9):e0163561. doi: 10.1371/journal.pone.0163561

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© 2016 Hamauchi et al

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 2 — Panel A shows representative photomicrographs of immunocytochemistry (CD144: Green, VEGFR2: Red, DAPI: Blue) in iPSCs 6 days after endothelial differentiation. Scale bar: 500 μm. Panel B shows the results of FACS analysis of CD144 and CD31 in the differentiated iPSCs. Sorted cells are gated and indicated as P5. Panel C shows the representative photomicrographs of immunocytochemistry for CD144 (green) and von Willebrand factor (vWF) after the purification and expansion of endothelial cells. Left: Control, right: MMD. Scale bar, 100 μm.