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. 2016 Aug 18;1(13):e84671. doi: 10.1172/jci.insight.84671

Figure 5. Pulse sequences used in this study.

Figure 5

(A) The 1D ge-HMQC (heteronuclear multiple quantum coherence) sequence is shown. The HMQC block was placed after PRESS (point resolved spectroscopy) localization. Two additional 13C inversion pulses were used to refocus the 13C chemical shift evolution. Minimum evolution time (t1) was 7.6 ms. In contrast to the conventional ge-HMQC sequence, 5 gradients were used for coherence selection. Gradients were applied in a ratio of 1:–1:–1:1:1. (B) The 1D ge-HSQC (heteronuclear single quantum coherence) sequence, based on STEAM (stimulated echo acquistion mode) localization. The inversion pulse during the t1, which was used on the 1H channel previously, was now applied on the 13C channel to again refocus 13C chemical shift evolution. Minimum t1 was 4.2 ms, and gradients were applied in a 2:–2:1 ratio. In both sequences, 1/2JCH was chosen as 3.95 (J = 127 Hz for CH2 lipids). No decoupling was applied.