| 10 μl | 10X NT buffer |
| 10 μl | 0.1 M β-mercaptoethanol |
| 10 μl | 10X nucleotide stock |
| 2 μl | E.Coli polymerase |
| 5 μl | DNase I dilution |
| 2.5 μl | 0.4 mM dTTP |
| 4 μl | 1 mM Biotin-16-dUTP or DIG-11-dUTP |
| 1 μg | template DNA |
| add sterile water to 100 μl | |
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