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. Author manuscript; available in PMC: 2016 Sep 23.
Published in final edited form as: Biochemistry. 2016 Sep 7;55(37):5272–5278. doi: 10.1021/acs.biochem.6b00649

Figure 3.

Figure 3

2D PDSD spectra of native and amyloid states of TTR with a contour level of 1.5 % obtained using various labeling schemes. A mixing time of 500 ms was used, unless indicated. (a) 13CO-His, 13Cα-Gly, and 13Cα-Ser. (b) 13CO-Ile, 13Cα-Met, and 13Cα-Ala labeled V30M amyloid PDSD spectra using mixing times of 100 ms (red) and 500 ms (blue) (c) 13CO-Ile, 13Cα-Met, and 13Cα-Ala labeled WT amyloid. (d) 13CO-Ile and 13Cα-Val labeled V30M amyloid. (e) 13CO-Gly and 13Cα-Val labeled WT (black) and V30M amyloid (red) with 1D slices at 60.5 ppm. * denotes spinning sidebands. The cross-peak marked by ? could not be assigned in this study.