Table 3.

Changes in marker gene expression in the placodes after injection of various constructs.

DOI: http://dx.doi.org/10.7554/eLife.17666.018

	Injection	Six1-MO*	Eya1-MO*	Six1-EnR	Eya1-mmMO	Six1-GR§	Eya1-GR§
	Phenotype	%	%	%	%	%	%
		(n)	(n)	(n)	(n)	(n)	(n)
Atoh1	Reduced	77**	90‡	94	10	26	42
		(26)	(20)	(18)	(21)	(19)	(12)
	Increased	0	0	0	0	35	42
		(26)	(20)	(18)	(21)	(17)	(12)
Gfi1a	Reduced	82†	67†	69	31	57	36
		(27)	(17)	(16)	(26)	(14)	(14)
	Increased	0	0	0	0	7	43
		(27)	(17)	(16)	(26)	(14)	(14)
Hes8	Reduced	74‡	83‡	70	17	60	57
		(35)	(35)	(46)	(24)	(40)	(56)
	Increased	0	0	24	0	15	29
		(35)	(35)	(46)	(24)	(40)	(56)
Hes9	Reduced	73‡	76‡	84	11	75	29
		(45)	(33)	(38)	(27)	(12)	(29)
	Increased	0	0	8	0	0	0
		(45)	(33)	(38)	(27)	(12)	(29)
Isl2	Reduced	66†	100‡	nd	27	50	24
		(38)	(17)	nd	(22)	(18)	(17)
	Increased	6	0	nd	0	31	41
		(38)	(17)	nd	(22)	(16)	(17)
Ngn1	Reduced	65‡	49†	84	17	17	36
		(51)	(43)	(31)	(24)	(30)	(59)
	Increased	0	16	6	4	23	41
		(51)	(43)	(31)	(24)	(30)	(59)
Pou4f1.2	Reduced	67‡	63†	71	16	47	81
		(48)	(30)	(35)	(19)	(15)	(37)
	Increased	0	0	0	0	13	0
		(48)	(30)	(35)	(19)	(15)	(37)
Sox2	Reduced	74‡	78‡	87	6	90	48
		(19)	(18)	(30)	(16)	(21)	(33)
	Increased	0	0	23#	0	0	12
		(19)	(18)	(30)	(16)	(21)	(33)
Sox3	Reduced	68‡	54†	39	9	49	40
		(25)	(26)	(31)	(22)	(25)	(23)
	Increased	0	0	71#	0	16	17
		(25)	(26)	(31)	(22)	(25)	(23)
Tlx1	Reduced	84†	91‡	100	33	40	7
		(31)	(32)	(13)	(15)	(10)	(15)
	Increased	6	0	0	0	40	73
		(31)	(32)	(13)	(15)	(10)	(15)

* Significant differences (Fisher’s exact test);.

^† p<0.05,.

^‡ p<0.001) to Eya1-mmMO injections are indicated.

^§ Dexamethasone treatment from stages 16–18 on.

^# Expression ectopic in epidermis.

n: Number of embryos analysed at both neural plate (stage 14–16) and tail bud (stage 21–26) stage.

nd: Not determined.