Figure 4. Frequency-doubling of pair-rule gene expression patterns is almost simultaneous and coincides with the first expression of the segment-polarity genes.
Each row shows the expression of a particular pair-rule gene or segment-polarity gene, while each column represents a particular developmental timepoint. Late phase 2 and early phase 3 both correspond to late Bownes stage 5; gastrulation is Bownes stage 6, and early germband extension is Bownes stage 7 (Bownes, 1975; Campos-Ortega and Hartenstein, 1985). All panels show a lateral view, anterior left, dorsal top. GBE = germband extension. The figure represents about 20 min of development at 25°C.
Figure 4—figure supplement 1. Relative expression of pair-rule genes during frequency-doubling.
Each row shows the relative expression of two pair-rule genes, while each column represents a particular developmental timepoint. Late phase 2 and early phase 3 both correspond to late Bownes stage 5; gastrulation is Bownes stage 6, and early germband extension is Bownes stage 7 (Bownes, 1975; Campos-Ortega and Hartenstein, 1985). All panels show lateral or ventrolateral views, anterior left, dorsal top. Single-channel images are shown in greyscale below each double-channel image (the channel listed first in the row label is always on the left). Each gene is shown as a different colour in the double-channel images. GBE = germband extension.
Figure 4—figure supplement 2. Relative expression of segment-polarity genes and pair-rule genes during frequency-doubling.
Each row shows the relative expression of a particular pair-rule gene and segment-polarity gene combination, while each column represents a particular developmental timepoint. Late phase 2 and early phase 3 both correspond to late Bownes stage 5; gastrulation is Bownes stage 6, and early germband extension is Bownes stage 7 (Bownes, 1975; Campos-Ortega and Hartenstein, 1985). All panels show a lateral view, anterior left, dorsal top. Single-channel images are shown in greyscale below each double-channel image (the channel listed first in the row label is always on the left). Each segment-polarity gene is shown in a different colour, while pair-rule gene expression is shown in red. GBE = germband extension.