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Measured and calculated values (µm) for the FWHM of the PSF in laser scanning confocal mode and wide-field epi-fluorescence mode for a conventional 5x/0.15 N.A. lens and the 4x/0.47 N.A. Mesolens used with oil immersion. We have assumed emission λ=550 nm for the calculations.
| 5x/0.15 N.A. | Mesolens (4x/0.47 N.A.) | |||
|---|---|---|---|---|
| Theoretical | Measured | Theoretical | Measured | |
| XY FWHM, epi | 1.9 | 1.9 | 0.6 | 0.7 |
| Z FWHM, epi | 41 | 51 | 6.3 | 7 |
| XY FWHM, confocal | 1.8 | 1.7 | 0.6 | 0.8 |
| Z FWHM, confocal | 25 | 38 | 3.7 | 8 |
