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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: J Neurovirol. 2016 Mar 23;22(5):615–625. doi: 10.1007/s13365-016-0435-6

Figure 3. Effect of Brd4 inhibitor JQ1 on JCV early transcription.

Figure 3

A. TC620 cells were transfected with luciferase reporter plasmid for the JCV early promoter together with vector plasmid or expression plasmid for Brd4 and treated with or without the active form of JQ1 (JQ1(+)) or its inactive isomer (JQ1(−)) at concentrations of 5 and 10μM. After 48 h, cells were harvested and assayed for luciferase activity as described in Methods. Activities were normalized to control without expression plasmid. The bar represents one standard deviation. B. Western blot for Brd4 expression in the experiment in Panel A. Grb2 was the loading control. C. To determine the effect of JQ1(+) and JQ1(−) on cell viability, cells were incubated with JQ1(+) at 5, 10 or 15μM for 24h (lanes 2, 4 and 6 respectively) or JQ1(−) at 5, 10 or 15μM for 24h (lanes 8, 10 and 12 respectively). An equal volume of DMSO was added to the odd numbered lanes corresponding to the amount for each following even numbered lane. Viabilty was measured by MTT assay.