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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: J Neurovirol. 2016 Mar 23;22(5):615–625. doi: 10.1007/s13365-016-0435-6

Figure 4. Effect of Brd4 and NF-κB p65 acetylation site mutants on JCV early transcription.

Figure 4

A. TC620 cells were transfected with vector or Brd4 expression plasmid with and without expression plasmid for NF-κB p65, wild-type or mutants K218R, K221R, K310R, K218Q, K221Q or K310Q. After 48 h, cells were harvested and assayed for luciferase activity as described in Methods. Activities were normalized to control without expression plasmid. The bar represents one standard deviation. B. The lysates from Panel A were analyzed by Western blot for expression of Brd4 and p65. The loading control was α-tubulin (α-Tub).