Table 3.
Characterization of SSR primers among different genotypes of Annona species
| Locus name | Sequence (5′–3′) | Fragment size (bp) | Number of alleles | Number of polymorphic alleles | Polymorphism (%) | PIC |
|---|---|---|---|---|---|---|
| LMCH-29 | F: GTACCATCTTTTAGGAAATC | 196–280 | 4 | 4 | 100 | 0.496 |
| R: TGCAATCTATGTTAGTCAC | ||||||
| LMCH-43 | F: CTAGTTCCAAGACGTGAGAGAT | 210–375 | 3 | 3 | 100 | 0.177 |
| R: ATAGGAATAAGGGACTGTTGAG | ||||||
| LMCH-48 | F: TTAGAGTGAAAAGCGGCAAG | 157–192 | 2 | 1 | 50 | 0.227 |
| R: TCAAGCTACAGAAAGTCTACCG | ||||||
| LMCH-70 | F: GAAGTTTTAGAGGCGATTCC | 152–178 | 3 | 3 | 100 | 0.254 |
| R: TTTTGCCACTTTACTGTCAC | ||||||
| LMCH-71 | F: AGATAACACCCGCCCACTAT | 282–497 | 3 | 2 | 66.67 | 0.169 |
| R: ACAACTTTTCTCCCAACCTATC | ||||||
| LMCH-78 | F: ATTTGATTGATTGATTTCCTA | 172–235 | 3 | 3 | 100 | 0.265 |
| R: CTTTTGCTTTCTTTCACATC | ||||||
| LMCH-79 | F: GAAGCAAGTAGACACGTAGTA | 212–384 | 5 | 5 | 100 | 0.694 |
| R: AGGGTTGGTATTTCTTTATAGT | ||||||
| LMCH-112 | F: TAACCCAGGATCTACAATAAT | 194–278 | 4 | 4 | 100 | 0.525 |
| R: TTGCATACATTTTCCTATTT | ||||||
| LMCH-114 | F: AAAATGTAGTGTGAAAGATGAC | 202–246 | 3 | 3 | 100 | 0.351 |
| R: GTCCATTCAGTTTTAAGTGC | ||||||
| LMCH-119 | F: CAGAAAATTAGCAGAGGACTCA | 191–287 | 3 | 3 | 100 | 0.265 |
| R: GTGGGTTGGGTTTTTAGGTC | ||||||
| LMCH-122 | F: AGCAAAGATAAAGAGAAGATAA | 190–212 | 3 | 3 | 100 | 0.310 |
| R: ATCCAAGCCTATTAACAACT | ||||||
| LMCH-128 | F: CTTGTTAAAATGGCTGTTACT | 252–288 | 3 | 3 | 100 | 0.343 |
| R: GCATTGAGCTGACATAACTC | ||||||
| Total | 39 | 37 | – | 4.068 | ||
| Average | 3.25 | 3.08 | 93.05 | 0.339 |