Table 3.

Association of CD3+, CD8+, CD45RO+ and FoxP3+ TILs in the tumor center and at the invasive margin with clinicopathological characteristics (significant values (P < 0.05) in bold)

	CD3+ TILs						CD8+ TILs
	Tumor center			Invasive margin			Tumor center			Invasive margin
	Low	High	P	Low	High	P	Low	High	P	Low	High	P
Age at surgery
<64	12	15	0.346	9	18	0.210	21	7	0.473	24	4	0.423
≥64	16	12		14	14		24	5		27	2
Sex
Male	15	17	0.480	12	20	0.444	24	9	0.177	29	4	1.000
Female	13	10		11	12		21	3		22	2
Localization of primary tumor
Colon	12	19	0.040	13	18	0.984	23	9	0.138	28	4	0.686
Rectum	16	8		10	14		22	3		23	2
UICC stage of primary tumor
I	1	3	0.815	0	4	0.165	3	1	0.511	3	1	0.292
II	6	6		4	8		13	1		14	0
III	15	12		15	12		20	7		23	4
IV	4	5		3	6		7	2		8	1
Unknown (n = 3)
Previous liver metastasis
No	17	22	0.090	18	21	0.309	30	10	0.315	35	5	0.657
Yes	11	5		5	11		15	2		16	1
DFI
<36 months	21	17	0.547	18	20	0.343	32	6	0.271	35	3	0.554
36–60 months	4	4		3	5		7	2		8	1
60 months	3	6		2	7		6	4		8	2
No. of pulmonary metastases
Singular	17	18	0.646	13	22	0.352	29	8	1.000	32	5	0.410
Multiple	11	9		10	10		16	4		19	1
Lymphatic vessel invasion
No	20	14	0.135	15	19	0.660	29	6	0.506	31	4	1.000
Yes	8	13		8	13		16	6		20	2
Chemotherapy before metastasectomy
No	4	7	0.281	3	8	0.326	10	2	1.000	11	1	1.000
Yes	24	20		20	24		35	10		40	5

	CD45RO+ TILs						FoxP3+ TILs
	Tumor center			Invasive margin			Tumor center			Invasive margin
	Low	High	P	Low	High	P	Low	High	P	Low	High	P
Age at surgery
<64	10	17	0.580	6	21	0.362	15	12	0.494	19	8	0.697
≥64	12	15		9	18		12	14		17	9
Sex
Male	11	20	0.361	10	21	0.393	16	15	0.908	22	9	0.573
Female	11	12		5	18		11	11		14	8
Localization of primary tumor
Colon	13	8	0.836	11	20	0.142	16	15	0.908	22	9	0.573
Rectum	9	14		4	19		11	11		14	8
UICC stage of primary tumor
I	2	2	0.891	0	4	0.453	1	3	0.228	3	1	0.393
II	4	9		2	11		4	8		6	6
III	11	15		8	18		17	9		20	6
IV	3	5		3	5		4	4		6	2
Unknown (n = 3)
Previous liver metastasis
No	14	24	0.369	9	29	0.333	16	21	0.088	23	14	0.172
Yes	8	8		6	10		11	5		13	3
DFI
<36 months	17	19	0.298	12	24	0.082	19	18	0.641	24	13	0.547
36–60 months	3	5		3	5		5	3		7	1
60 months	2	8		0	10		3	5		5	3
No. of pulmonary metastases
Singular	15	21	0.845	10	26	1.000	14	22	0.011	22	14	0.122
Multiple	7	11		5	13		13	4		14	3
Lymphatic vessel invasion
No	14	19	0.752	11	22	0.253	17	15	0.695	25	7	0.050
Yes	8	13		4	17		10	11		11	10
Chemotherapy before metastasectomy
No	6	6	0.517	3	9	1.000	4	7	0.277	7	4	0.730
Yes	16	26		12	30		23	19		29	13

DFI disease-free survival to first pulmonary metastasis