Fig. 2.

RNA oligonucleotides corresponding to specific 3′- and 5′-UTR sequences of the human and rat I insulin mRNA. In (A), the different RNA-oligonucleotides used in the Dynabead pull down experiments are shown. Oligonucleotides representing different regions of the human and rat 3′- and 5′-UTRs were biotinylated so that RNA binding proteins could be affinity purified using streptavidin coated dynabeads. Boxes represent pyrimidine rich sequences with potentially regulatory roles for mRNA stability and translation. The numbering of the first and last nucleotides corresponds to their position on the mRNA according to their NCBI accession number, NM_000207.2 and NM_019129.3 respectively. In (B), a simplified representation of the predicted interaction between the two long rat oligonucleotides is shown.