PCM1 depletion results in reduced GBM cell proliferation due to increased apoptotic cell death. (A, D) Average cell numbers per well 5 days after plating for L0 (A) and S3 (D) cell lines derived from PCM1-positive control clones (open-filled bars) or PCM1-depleted clones (black-filled bars). (B, E) Percentages of cells from control or PCM1-depleted clones that were Ki67+, PHH3+, or BrdU+ for L0 (B) and S3 (E) cell lines 5 days after plating. (C, F) Percentages of cleaved caspase 3–positive immunolabeled cells detected by FACS and percentages of Trypan blue–labeled cells 5 days after passaging for L0 (C) and S3 (F) cell lines. *P < .05, **P < .01, ***P < .001, Student's t test.