Figure 2.

Phenotypic correction of LAD-I lymphoblastoid cells. LAD-I and HD LCs were transduced with hCD18-LVs and control eGFP-LVs at an MOI of 10 i.u./cell. (A) PMA-induced aggregation assays. Cells were incubated with either PBS or PMA and let to aggregate for 1 hr. Anti-CD18 blocking antibody was also added to demonstrate that the observed aggregation was dependent on hCD18. LV:Chim.hCD18 was used at two different MOIs: low MOI (LM 10 i.u./cell) and high MOI (100 i.u./cell). (B) Soluble ICAM-1 binding assay. LFA-1 integrins were activated and thus cells were let to bind to sICAM-1 in the presence or absence of Mg²⁺ and EGTA for Ca²⁺ chelation. Chim LV was used at two different MOIs: low MOI (LM 10 i.u./cell) and high MOI (100 i.u./cell). The significance of differences between groups is expressed as *p < 0.05 and **p < 0.01. PBS, phosphate buffered saline; PMA, 4 beta-phorbol-12-myristate-13-acetate. See Supplementary Table S1 for list of antibodies used.