FIG 7.

Capacity of NGS to detect mixed-genotype/subtype samples. Observed ratios of NGS read counts between component genotypes genotype A (Gt A) and genotype B (Gt B) (y axis) compared to their input ratios (x axis), plotted on a log/log scale. The dotted line represents the expected position of data points if the assays were able to detect both input genotypes (genotypes A and B) with equal efficiency. Samples of mixed genotype of known ratio (the input ratio) were acquired from QCMD or through patient samples or in vitro transcripts of known genotype that were mixed in vitro (listed in Table S1B in the supplemental material).