TABLE 2.
Turnaround times and costs associated with norovirus genotyping by PCR and Sanger sequencing versus SureSelect target enrichment full-genome sequencing
| Genotyping method | Hands-on time (h) | Total turnaround time (days) | Reagent costs per sample (£) |
|---|---|---|---|
| PCR and Sanger sequencinga | 7 | 3 | 32 |
| Full-genome sequencing with SureSelect target enrichment | 11.5 | 6 | 86–93b |
a
PCR amplification of three sites of interest for norovirus genotyping, i.e., RNA-dependent RNA polymerase, capsid shell domain, and capsid P2 domain, including one round of nested PCR, assuming that the RNA-dependent RNA polymerase and capsid shell domain targets are amplified and sequenced simultaneously.
b
Costs based on batches of 96 or 48 samples and sequencing with an Illumina MiSeq system.