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. 2016 Sep 23;16:73. doi: 10.1186/s12935-016-0349-7

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© The Author(s) 2016

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Hypoxia upregulates ANRIL expression in osteosarcoma cells. a Expression level of ANRIL by qRT-PCR in 15 osteosarcoma tissues and paired non-tumor tissues. GAPDH was used as a loading control. b ANRIL expression under both normoxic and hypoxic conditions were analyzed by real-time PCR. c ANRIL promoter activity under both normoxic and hypoxic conditions were analyzed by luciferase assay (**, P < 0.01, n = 3). β-actin was used as the internal control (**, P < 0.01, n = 3)