Skip to main content
. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Methods. 2016 Apr 20;108:56–64. doi: 10.1016/j.ymeth.2016.04.014

Figure 6.

Figure 6

Rim1-Pif1 heterodimer detected with the SDA cross-linker (reproduced from [41], an open access publication). Rim1 and Pif1 proteins were cross-linked with SDA using a 2-step procedure. The reaction products were resolved on 5–15% gradient SDS-PAGE. Open triangle marks the position of Rim1-Pif1 heterodimer. Filled triangle marks the position of Rim1-Rim1 homodimer. Arrow points to the position of Pif1 bound by two molecules of Rim1. A, Rim1 and Pif1 without cross-linker. The Pif1 and Rim1 isoforms used in this work localize at 60 kD and 15 kD regions, respectively. B, lanes 1–3, Pif1 was reacted with SDA first, followed by addition of Rim1 and UV irradiations. C, lanes 1–3, Rim1 was reacted with SDA first, followed by the addition of Pif1 and UV irradiation. D, lane 1 – same reaction conditions as in C, lane 2 – ssDNA (T70) was added prior to cross-linking with SDA. UV exposure times at the second step of cross-linking are indicated, in minutes.