Table 2.

Gene expression in aortas from ApoE^−/− and DKO mice fed a Western diet

Target gene	ApoE−/− female	DKO female	ApoE−/− male	DKO male
IL-6	1.00±0.45	1.37±0.80	1.00±0.44	0.08±0.04
IL-18	1.00±0.60	0.87±0.44	1.00±0.34	1.269±0.23
TNFα	1.00±0.64	0.18±0.13	1.00±0.60	0.30±0.06
ICAM1	1.00±0.25	0.28±0.18	1.00±0.23	0.15±0.04*
VCAM1	1.00±0.26	0.29±0.15	1.00±0.23	0.57±0.27
MCP1	1.00±0.25	0.99±0.72	1.00±0.05	0.73±0.33
CXCL1	1.00±0.09	0.32±0.17*	1.00±0.33	0.20±0.07*
AT1A	1.00±0.32	0.85±0.47	1.00±0.69	0.23±0.07
AT2	1.00±0.13	0.33±0.24	1.00±0.88	0.42±0.15
MMP2	1.00±0.33	0.64±0.40	1.00±0.42	0.31±0.12
MMP9	1.00±0.14	5.84±2.90	1.00±0.33	2.00±1.47
MMP14	1.00±0.53	0.52±0.38	1.00±0.33	0.66±0.16
TIMP1	1.00±0.11	0.53±0.57	1.00±0.10	0.40±0.07*
TRAF3IP2	1.00±0.34	-	1.00±0.23	-
RECK	1.00±0.40	0.73±0.31	1.00±0.13	0.25±0.05*
ADAM10	1.00±0.82	7.40±5.20	1.00±0.053	1.00±0.25
ADAM17	1.00±0.25	0.57±0.37	1.00±0.06	0.23±0.04*

IL-6, interleukin-6; IL-18, interleukin-18; TNFα, tumor necrosis factor α; ICAM1, intercellular adhesion molecule 1; VCAM1, vascular cell adhesion molecule; AT1A, angiotensin II receptor, type 1a; AT2, angiotensin II receptor, type 2; MMP, matrix metalloproteinase; TIMP1, tissue inhibitor of matrix metalloproteinase 1; TRAF3IP2, TRAF3-Interacting Protein 2; RECK, reversion-inducing cysteine-rich protein with kazal motifs; ADAM, a disintegrin and metalloproteinase. 18S rRNA served as the endogenous invariant control, and relative gene expression in ApoE^−/− female mice were taken as ‘1’. DKO; double knockout mice (TRAF3IP2^−/−/ApoE^−/−). Values are mean ± SEM (n = 3–5).

^*indicates significance at p<0.05 when compared between ApoE^−/− and DKO mice belonging to the same gender. Statistical significance was calculated by Student’s t-test.