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. Author manuscript; available in PMC: 2017 Sep 1.
Published in final edited form as: J Thromb Haemost. 2016 Aug 6;14(9):1822–1832. doi: 10.1111/jth.13390

Figure 3. Higher Pg levels are associated with decreased BBB breakdown and reduced vascular MMP-9 expression after stroke.

Figure 3

(A) Leakage (arrows) of albumin (red) into the brain parenchyma outside collagen IV-stained (green) blood vessels was assessed in the ischemic hemispheres by comparison to the non-ischemic hemispheres of Pg−/−, Pg+/− and Pg+/+ mice. Merged images (20x) show areas of overlapping staining for collagen IV and albumin (yellow) and DAPI-stained nuclei in blue. (B) Representative immunofluorescence images (20x) showing MMP-9 expression (red) in the ischemic hemispheres of Pg−/−, Pg+/− and Pg−/− mice as detected by goat anti-mouse MMP-9 primary antibody followed by donkey anti-goat DyLight® 549. (C) The bar graphs show the ratio of the total area of albumin staining (in 20x fields) and (D) MMP-9 fluorescence intensity in the ischemic vs. the non-ischemic hemisphere of each mice group (mean ± SE). n= 4-5 per group, *p<0.05, **p<0.01, ns (not significant).