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. Author manuscript; available in PMC: 2017 Oct 1.
Published in final edited form as: Exp Hematol. 2016 Jun 27;44(10):975–981. doi: 10.1016/j.exphem.2016.06.249

Fig. 3.

Fig. 3

Inhibition of ROS with low dose of ROS scavenger does not affect differentiation. (A-B) Flow cytometric analysis of differentiation (A) and apoptosis (B) of Ter119 negative mouse fetal liver erythroblasts cultured in Epo (2 units/ml) containing medium in the presence of different concentration of NAC for 48 h. Statistical analysis with student's t-test is on the right. (C) Hemoglobin concentration of the indicated cells in panel (A). An equal number of the cells were collected. Total hemoglobin was quantified by measuring light absorbance at 540 nm using Drabkin's reagent. All data were obtained from three independent experiments and presented as mean±SD. *** p <0.0001.