ADAR1 is essential for definitive erythropoiesis in transplanted adult mice. (A) Schematic representation of transplant experiments. PB enumeration of RBCs (B) and hemoglobin (C), hematocrit (D), mean corpuscular volume (MCV) (E), chimerism (CD45.2+) (F), and serum Epo levels (G) at 16 weeks after transplantation. BM cellularity (H), enumeration of erythroid progenitors (I), and allele distribution (determined by gDNA semi-qPCR) (J). (K) Spleen weight, cellularity, and chimerism. Representative FACs plots of Ter119+ erythrocytes (L) and quantitation of mature erythroid fractions (M) determined by size and CD44 expression. Results are mean ± SEM (Δ/+ n = 4 and Δ/− n = 7, pooled from two experiments). *p < 0.05 and **p< 0.005 compared with Δ/+.