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. Author manuscript; available in PMC: 2017 Oct 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2016 Aug 14;309:15–23. doi: 10.1016/j.taap.2016.08.011

Figure 4.

Figure 4

Relationship between time of TCDD addition and in vitro IgM response. SKW-AHR+ (A), mouse (B) and rat (C) primary B cells were activated with PWM (150 μg/ml and 15 μg/ml respectively) and treated with 30 nM TCDD and/or vehicle (0.01% DMSO) at indicated time-points after activation. Supernatants were harvested at 120 h post PWM activation and the amount of secreted IgM was measured by sandwich ELISA. The data are representative of three separate experiments (one animal per experiment) with 4 experimental replicates per group. Statistical significance was determined using Dunnett's two-tailed t test; * represents values that are significantly different from naive at p < 0.05.