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. Author manuscript; available in PMC: 2017 Oct 15.
Published in final edited form as: Toxicol Appl Pharmacol. 2016 Aug 14;309:15–23. doi: 10.1016/j.taap.2016.08.011

Figure 6.

Figure 6

Time-dependent induction of the Cyp1A2 (A) and Cyp1B1 (B) gene expression by TCDD in SKW clones. The SKW cells (5×106/ml) were treated with 30 nM of TCDD or 0.01% DMSO (VH) for the indicated periods of time. Total RNA was isolated, and steady-state Cyp1A2 and Cyp1B1 mRNA levels were measured by TaqMan qRT-PCR. Samples were normalized to the endogenous 18S ribosomal RNA, which served as loading control. Fold induction values were calculated relative to time-matched vehicle controls. The data are representative of two separate experiments with three experimental replicates per group. *, values significantly different (p<0.05) from time-matched vehicle controls.