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Effects of the R554K SNP on the AhR protein stability. Time-dependent AhR protein degradation in SKW-AHR+ and R554K cells at 0, 4, 8 and 24h post TCDD treatment was examined by Western Blotting. Cell lysate protein (80 μg) was loaded in each lane, resolved on 4-20% SDS-PAGE gel and probed with anti-AhR antibody. Results are representative of more than three separate experiments.
