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. 2016 Aug 14;139(10):2751–2765. doi: 10.1093/brain/aww205

Figure 4.

Figure 4

Reduced CYFIP2 expression leads to increased αCaMKII expression at protein but not mRNA level, and increased tau phosphorylation in the hippocampus. ( A ) Representative western blots. ( B ) Quantification showed that αCaMKII protein expression is significantly upregulated ∼2-fold in hippocampal synaptosomes of Cyfip2+/− mice (black bar) in comparison to wild-type littermates (grey bar; n = 8 per genotype). ( C ) Quantitative PCR analysis showed that Camk2a /αCaMKII mRNA levels are not elevated in hippocampus of Cyfip2+/− mice (black bar) in comparison to wild-type littermates (grey bar; n = 8 per genotype). ( D ) Representative western blots. ( E ) Quantification showed an ∼50% increase in phosphorylation of tau at serine-214 in hippocampal synaptosomes of Cyfip2+/− mice (black bar) in comparison to wild-type littermates (grey bar; n = 8 per genotype). ( F ) Quantification showed that total levels of tau did not differ in hippocampal synaptosomes of Cyfip2+/− mice (black bar) in comparison to wild-type littermates (grey bar; n = 8 per genotype). Means ± SEM are shown. * P < 0.05, ** P < 0.01.