Skip to main content
. 2016 Aug 31;113(38):10631–10636. doi: 10.1073/pnas.1524490113

Fig. 2.

Fig. 2.

DNMT3a KO mice have evidence of improved CD8+ T-cell memory. (A) Absolute number of gp33–41-tetramer+CD8+ T cells in the spleen of WT or DNMT3a KO littermate mice 60 d post-LCMV infection. Bar graphs (mean ± SEM) compare WT (n = 4) and DNMT3a KO (n = 4) mice. (B) IFN-γ production after ex vivo gp33–41 peptide stimulation from splenocytes from day 60 LCMV-infected mice. Bar graphs (mean ± SEM) compare WT (n = 4) and DNMT3a KO (n = 4) mice. (C) Splenocytes from 6-mo-old littermate WT or DNMT3a KO mice were stained for markers of central memory T cells. Representative plots of CD62L versus CD44 are gated on CD8+ T cells. Bar graph (mean ± SEM) compares WT (n = 9) and DNMT3a KO (n = 13) mice. All experiments in A–C were performed three times with similar results. For all panels, *P < 0.05, **P < 0.01, ****P < 0.0001 (unpaired two-tailed Student’s t test).