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. 2016 Aug 31;113(38):10631–10636. doi: 10.1073/pnas.1524490113

Fig. S3.

Fig. S3.

DNMT3a expression is dispensable for acute CD8+ T-cell function in response to viral infection. (A) Cohorts of DNMT3a WT or KO mice were infected with VacOva and T-cell lytic function was measured 7 d later by in vivo lysis of adoptively transferred eFluor 450high-labeled Ova257–264-pulsed splenocytes and eFluor 450low-labeled control splenocytes. Bar graph (mean ± SEM) compares specific lysis in WT (n = 10) and DNMT3a KO (n = 6) mice. This experiment was performed three times with similar results. (B) WT and DNMT3a KO mice were infected with influenza PR8 intranasally and followed for weight loss and survival. Mean ± SEM weight versus day postinfection is shown. This experiment was performed two times with similar results. (C) Cytokine production (mean ± SEM) 10 d postinfluenza PR8 infection from lung-resident lymphocytes measured by intracellular cytokine staining 6 h after stimulation with T2-Db cells presenting NP366–374-peptide. N = 5 mice per group. This experiment was performed three times with similar results. n.s. = P > 0.05 (unpaired two-tailed Student’s t test).