Skip to main content
. 2016 Aug 31;113(38):10631–10636. doi: 10.1073/pnas.1524490113

Fig. S5.

Fig. S5.

DNMT3a loss does not affect CD8+ early effector cell expression of critical transcription factors or IL2ra. Expression of indicated transcription factors/key genes was measured by: (A) Quantitative PCR of cDNA from adoptively transferred OT1 DNMT3a WT and KO early effector cells sorted 4 d after VacOVA infection, normalized to Hprt expression. Bar graphs show the mean ± SD of expression from cells sorted from individual mice. (WT n = 10, DNMT3a KO n = 9 individual mice). No significant differences were seen between the groups. (B) Nanostring analysis of RNA from adoptively transferred WT and DNMT3a KO OT1 early effector cells sorted 4 d after VacOva infection (mean ± SD, n = 3 individual mice per group). No signficant differences were observed between the groups. (C) Flow cytometry gated on CD8+ gp33–41-specific CD127−KLRG1− early effector cells from WT and DNMT3a KO mice 5 d following LCMV infection. Indicated proteins were detected by intracellular staining after fixation and permeabilization. (MFI ± SEM, n = 3 mice per group). *P < 0.05.