Table S2.
CT values and geometric means of housekeeping genes
Gene | Basal | Pilocarpine | ||
Microglia | Monocytes | Microglia | Monocytes | |
HPRT1 | 32.3 ± 0.5 | 34.9 ± 0.3 | 34.2 ± 0.5 | 34.5 ± 0.6 |
β-Actin | 28.7 ± 0.3 | 29.7 ± 0.6 | 30.5 ± 0.8 | 30.6 ± 0.6 |
GAPDH | 23.0 ± 0.3 | 22.7 ± 0.5 | 24.3 ± 1.0 | 22.3 ± 0.9 |
Geometric mean | 28.0 ± 0.3 | 28.8 ± 0.4 | 29.4 ± 0.8 | 28.7 ± 0.6 |
Data are the mean ± SEM in four groups of mice (n = 5 microglia basal, n = 5 monocytes basal, n = 9 microglia pilocarpine, and n = 5 monocytes pilocarpine) for 4-d inflammatory mediator measurement in myeloid cell subsets.