Table 2.
Genes affected by Act D or RG7787 using apoptotic array
| KLM1 | RH16 | |||||
| Symbol | Act D | RG7787 | Comb | Act D | RG7787 | Comb |
| TNFα | 10 | 147.6 | 103.1 | 1.2 | 6.9 | 11.2 |
| TNFβ | 1.2 | 7.5 | 13.4 | 4.8 | 2.9 | 3.5 |
| TNFSF7 (CD70) | 2.7 | 4.1 | 3.3 | 1.6 | 1 | 2.8 |
| Fas | 1.1 | 2.4 | 1 | 5.9 | 0.1 | 6.1 |
| TRAILR2/DR5 | 2.7 | 8.3 | 4.6 | 2 | 1 | 3.9 |
| TNFR2 | 10.5 | 15.5 | 8.1 | 0.5 | 0.6 | 0.4 |
| TNFRSF9 (CD137) | 4.6 | 68.8 | 15.7 | 0.7 | 4.5 | 2.8 |
| TNFRSF7 (CD27) | 3.4 | 0.2 | 0.2 | 2.4 | 0.5 | 1.8 |
| Caspase-1 | 0.7 | 0.3 | 0.4 | 15 | 1.3 | 13.5 |
| Caspase-10 | 2.1 | 1.9 | 3 | 3.6 | 1.5 | 5.4 |
| Caspase-3 | 2.9 | 1.4 | 3.8 | 1.8 | 1.6 | 2.4 |
| Caspase-5 | 2.8 | 0.7 | 5.6 | 2.8 | 1.3 | 3.5 |
| Caspase-7 | 3.4 | 1.1 | 2.2 | 1.2 | 0.9 | 1.8 |
| Caspase-9 | 1.9 | 2.4 | 3.2 | 1 | 3.4 | 2.4 |
| Bcl-10 | 2.2 | 4.3 | 3.2 | 2.3 | 1.9 | 3.9 |
| BCL2A1 | 3.4 | 7.4 | 21.6 | 2.1 | 19.2 | 22.6 |
| Bim | 3.1 | 0.7 | 2.3 | 1.8 | 0.7 | 1.7 |
| Bik | 1.8 | 6.1 | 3.6 | 2.5 | 1.1 | 4.1 |
| CIAP2 | 4.7 | 46.3 | 13.2 | 8.6 | 5.7 | 9.8 |
| GADD45A | 4.1 | 63.1 | 30 | 4.5 | 4.1 | 21.2 |
| TP53 | 2.4 | 5.6 | 8.8 | 1.5 | 1.1 | 1.6 |
| TP53BP2 | 1.6 | 5.8 | 3.7 | 1.6 | 1.7 | 1.8 |
| RIPK2 | 2.3 | 2.9 | 2.2 | 3.1 | 2.4 | 4.8 |
KLM1 or RH16 cells were treated with Act D (KLM1, 10 ng/mL; RH16, 5 ng/mL) or RG7787 (KLM1, 100 ng/mL; RH16, 200 ng/mL) for 24 h. Then total RNA was isolated and apoptotic array performed. The numbers are generated using web-based data analysis (Qiagen) by setting the untreated control as 1 and using ACTB, B2M, GAPDH, HPRT1, and RPLP0 as the internal control. The genes that changed over threefold were selected and presented in the table.